ISSN: 0125-2364, Online ISSN: 2586-9531

Volume 41 No.4 October - December 2013


P.797-807

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Medicinal Plants of the Genus Dioscorea L. Used in Traditional Thai Medicine Prescriptions

สมุนไพรจากพืชสกุลกลอยในตารับยาแผนไทย

เกศริน มณีนูน

The genus Dioscorea L. is distributed throughout the world and widely used in terms of  food and medicinal plants. In Thailand, 42 species of Dioscorea L. have been revised. Revision results mention that this genus plays an important role as dietary plants. However, little study of the genus has been done in terms of medicinal uses. Three species of the genus, Dioscorea bulbifera L., D. hispida Dennst. and D. membranacea Pierre ex Prain and Burkill, are used in traditional medicine. Only one species, D. membranacea, has been reported. This review focuses on the uses of the genus Dioscorea which was reported in traditional medicine including related researches and further studies.


P.808-822

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The Peculiar Plants of Podostemaceae in Na Haeo District, Loei Province

พืชที่มีลักษณะเฉพาะของวงศ์ Podostemaceae ในอาเภอนาแห้ว จังหวัดเลย

เพ็ชรรัตน์ เวฬุคามกุล และ ละออ อัมพรพรรดิ์

The Podostemaceae is the largest family of aquatic angiosperms. Because of the unusual morphology of the plant body, it is difficult to demarcate into stem and root. Doublefertilization and endosperm production, two defining features of angiosperms, do not occur in the family. They grow only in open, sunlit areas, attaching to rocks or hard substrata in beds of rivers, rapids or waterfalls that are subject to seasonal changes in water level. The vegetative plants are immersed in rapidly flowing water in the rainy season. As the water level recedes towards the close of the rainy season, the plants form flowers, which open as exposing to air. Fruits and seeds quickly develop. The plants will soon die if they are placed in standing water or in muddy substratum. If the environmental changes cause high water level or drought all year round, the plants would be incapable of existence and finally might be lost. Podostemaceae consists of three subfamilies which are Podostemoideae, Tristichoideae and Weddellinoideae. The subfamily Weddellinoideae is not present in Thailand. Approximately 54 genera and 300 species worldwide are distributed in the tropical and subtropical regions. Most of them are endemic. Out of 47 species, thirty-four species and 3 varieties are endemic in Thailand. In Na Haeo district, Loei province Hydrobryum tardhuangense M. Kato and H. loeicum M. Kato grow in rapids and waterfalls along Hueang River. The former species is also distributed in Lao PDR and considered to be threatened while the latter species is endemic to Thailand and considered to be endangered according to the conservation status. 


P.823-832

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Symbiotic Seed Germination of Orchids

การเพาะเมล็ดกล้วยไม้แบบสมชีพ

สุรีย์พร นนทชัยภูมิ

Orchids are economically important plants. Moreover, majority of native Thai orchids are rare or endangered. Seed germination is a standard technique for propagation of orchid hybrids to select desirable traits and for propagation of orchid species recalcitrant to tissue culture. Symbiotic seed germination, i.e., germination of seeds by co-culturing with orchid mycorrhizal fungus, is an option of orchid seed germination. Comparative studies between in vitro asymbiotic and symbiotic seed germination demonstrated that symbiotic method gave superior results compared to asymbiotic method because mycorrhizal fungus-infected seeds absorbed water better than non-infected seeds. Moreover, protocorms from symbiotic method could develop more rapidly than protocorms from asymbiotic method. This article is aimed to review current knowledge concerning symbiotic orchid seed germination including physiology of orchid seeds, orchid mycorrhizal fungi, symbiotic germination methods, and factors influencing symbiotic orchid seed germination. Understanding the requirements for symbiotic seed germination will assist the improvement of this technique to be used for commercial orchid propagation and conservation.


P.833-844

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Theoretical Study of Gas Adsorption on Metal Doped Single Wall Carbon Nanotube

การศึกษาเชิงทฤษฎีของการดูดซับแก๊ส บนท่อนาโนคาร์บอนแบบผนังเดี่ยวที่มีการเติมโลหะ

ฌาณุกรณ์ ทับทิมใส

Carbon nanotubes are novel nanomaterials which have the excellent mechanical, thermal and electronic properties. High surface area of carbon nanotubes can be developed to be material for clean energy storage or toxic gas sensor. The theoretical study of gas adsorption on carbon nanotube is reviewed. The results reveal that gas can absorb on defected carbon nanotube stronger than that of pristine carbon nanotube. Metal doping on carbon nanotubes can improve the gas adsorption abilities of the tubes. Moreover, the structural and electronic properties of gas adsorption on pristine and metal doped carbon nanotubes are also reported.


P.845-858

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Surface Modifications of Nanoparticles for Biomedical Application

การปรับแต่งพื้นผิวอนุภาคนาโนเพื่อการประยุกต์ใช้ในทางชีวการแพทย์

เฉลิมชัย ปิละพงค์

Applications of nanoparticles in biomedical science have been studied extensively because of their potentials to bring tremendous changes in diagnostic and treatment for many diseases. Firstly, however, surface of nanoparticles have to be modified in order to improve their physicochemical properties. In this review, it starts with a brief introduction regarding water solubilization of nanoparticles, followed by example approaches to solubilize them into aqueous solution such as ligand exchange, silica coating and polymer coating. Next, bioconjugate chemistry between nanoparticles and specific biomolecules was mentioned briefly. Finally, the selected biomedical applications of nanoparticles were also mentioned.


P.859-872

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Gold Nanostructures: Synthesis and Applications for Cancer Therapy

โครงสร้างระดับนาโนของทอง: การสังเคราะห์และการประยุกต์ใช้ในการรักษาโรคมะเร็ง

อภิวัฒน์ ชมภูสอ

Gold nanostructures have been used in a broad range of biological and biomedical applications due to their brilliant optical properties. This review provides the synthesis of gold nanostructures for examples nanospheres, nanorods, nanoshells, and nanocages. By engineering the size, shape and structure of gold, the surface plasmon resonance can be tailored from the visible to the near infrared region. In the second part, cancer therapies that take benefit of the radiative properties resulting from surface plasmon oscillation are presented. Tunable optical properties coupled with the inertness of gold make them an excellent platform for biomedical applications.


P.873-884

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Development of Paper-Based Biosensors for Point of Care Diagnostics

การพัฒนาไบโอเซนเซอร์แบบกระดาษสู่ระบบการวินิจฉัยโรคใกล้ชิดผู้ป่วย

ภัทรสุดา รักทอง และ รินา ภัทรมานนท์

The natural paper products are used in various fields including diagnostics by applying the properties to match with the interface of biosensors, the highly effective device for analyzing biological samples. Paper-based biosensors are developed as a tool for analyze and diagnose a wide range of biological compounds by modifying the recognition element and transducer that can be combined for qualitative or quantitative analysis. They are also portable and are able to use outside the laboratory and for the field study. Some paper-based biosensors have been engineered for patients to read the result on their own at home. The diagnosis can be reported by computer software or mobile applications. Paper-based biosensor might replace the original equipments that are large, expensive, and imported from abroad, and requires an expert to read the analysis. Paper-based biosensors are also useful for point of care diagnosis system that can be accessed in the remote or isolated regions for proper healthcare.


P.885-898

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Fluorometabolite Natural Products

สารผลิตภัณฑ์ธรรมชาติเมแทบอไลต์ฟลูออรีน

พิทักษ์ นาสมใจ

Fluorine is ranked 13th most abundant element in the Earth’s crust and the most abundance halogen. However, fluorometabolites are exceedingly rare and only 15 compounds have been officially identified confining in plants and two bacteria. In this article the discovery, biosynthetic pathways, and bioactivities of fluorometabolites are reviewed. In particular, the most advanced study in bacterium Strptomyces cattleya is highlighted. The discovery of Fluorinase enzyme and its application as a synthetic tool for instalment of a C-F bond to organic molecules is also discussed.


P.899-907

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Legendre’s Function and Additional Solution of a Weighted Heat Equation

ฟังก์ชันเลอช็องดร์กับการหาผลเฉลยของสมการความร้อนถ่วงน้ำหนัก

ประภาส ผิวอ่อน

In the article, a new simple method for solving solution of a weight heat equation is presented. The method bases on recursive relation and some property of Legendre’s function. Proof of the relation is also discussed.


P.908-918

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Adjoint Matrix and Inverse Matrix of Semi-Magic Squares

เมทริกซ์ผูกพันและเมทริกซ์ผกผันของกึ่งจัตุรัสกล

นิรุต มีเกิด

A semi-magic square is a square matrix whose row and column sums are all equal to the same constant m. In this paper, we prove that if A is a semi-magic square whose the sum is m  0 and det (A)  0, then adj (A) and A-1 are semi-magic squares whose the sum are det (A)/m and 1/m, respectively.


P.919-927

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Generalized Beauty:Numbers of Digits as 1 at All and Linear Equations

ความสวยงามวางนัยทั่วไป: จำนวนที่ทุกหลักเป็นเลข 1 และสมการเชิงเส้น

อัยเรศ เอี่ยมพันธ์

The main purpose of this article is to study and find a general form and a relationship between numbers of digits as 1 at all and linear equations which constant is the number of all digit in numbers of digits as 1 at all.


P.928-935

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Receptacle Structure of Termite Fungus (Termitomyces)

รูปร่างของรีเซพเทเคิลในเห็ดปลวก

เมธี ศรีไชยวงศ์   อุษา กลิ่นหอม และ ขวัญเรือน บุตรสาทร พาป้อง

The receptacle is a structure which connects between pseudorhiza and fungus comb.The genus Termitomyces has an important structure which is called receptacle. The detail of receptacle structure has not been reported. The aim of this study to examine the structure of receptacle in genus Termitomyces. One hundred and nineteen samples of Termitomyces were collected from dry dipterocarp and community forests in Northeast of Thailand. The result shows that there were 5 types of receptacle including rod shape, white radish shape, taro shape, lace shape and knob shape.


P.936-944

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Cytogenetics of I-san fighting fish (Betta smaragdina Ladiges, 1972)

พันธุศาสตร์เซลล์ของปลากัดอีสาน (Betta smaragdina Ladiges, 1972)

วิไลลักษณ์ เครือเนตร และ ณัฎฐา นิธิกุลวรวงศ์

The first Cytogenetics of I-san fighting fish (Betta smaragdina Ladiges, 1972) at Phuhinladchorfar canal, Nong Bua Lam Phu province was investigated in this study. Mitotic chromosome was prepared directly from kidney of specimens after In vivo colchicines treatment. The metaphase spreads were performed on microscopic slides and air-dried. Conventional staining technique was applied to stain the chromosome with Giemsa’s solution 20%. The results showed that the number of diploid chromosome of I-san fighting fish was 2n = 42, fundamental number (NF) was 50 in both male and female. The types of chromosomes were 6 large acrocentric, 4 large telocentric, 2 medium acrocentric, 20 medium telocentric and 10 small telocentric chromosomes. The karyotypes are not different between sex chromosomes male and female. The karyotype formula for the I-san fighting fish is as follow:

2n (42) = L6a+ L4t+M2a+  M20t+ S10t


P.945-953

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Diversity of Trees in Hin Chang Si, Nam Phong National Park, Nong Ruea District, Khon Kaen Province

ความหลากหลายของพรรณไม้ต้นบริเวณหินช้างสี อุทยานแห่งชาติน้ำพอง อำเภอหนองเรือ จังหวัดขอนแก่น

พันธ์ทิวา กระจาย   วิษณุ สายศร   ประนอม จันทรโณทัย   วรพล ดีปราสัย และ พิมพ์วดี พรพงศ์รุ่งเรือง

A survey and taxonomic investigation of tree species along the nature trail at Hin Chang Si, Nam Phong National Park, Nong Ruea district, Khon Kaen province was carried out between January 2010 and December 2011. Seventy seven species, 61 genera and 33 families were enumerated. Due to lack of flowering or fruiting materials, which are the most important part for species identification, five specimens and two specimens can only be identified at generic and familial levels, respectively. The largest family is Leguminosae, with 13 genera and 15 species. The second largest family is Euphorbiaceae, with six genera and eight species.


P.954-966

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Screening of Cellulolytic Microorganisms for Stimulating of Rice (Oryza sativa L.) and Sweet Corn (Zea mays L. var. saccharata) Seed Germination

การคัดแยกจุลินทรีย์ย่อยสลายเซลลูโลสเพื่อกระตุ้นการงอกของเมล็ดพันธุ์ข้าว (Oryza sativa L.) และข้าวโพดหวาน (Zea mays L. var. saccharata)

ทิพย์นภา วงษ์คูณ   โสภณ บุญลือ และ นันทวัน ฤทธิ์เดช

Isolation of cellulolytic microorganisms from soil, filter cake and distillery samples was made. The isolated cellulolytic microorganisms were studied for the stimulating of seed germination. It was found that 26 isolates of bacteria, 4 isolates of actinomycetes and one isolate of fungus degraded carboxy methyl cellulose on CMC agar. The hydrolysis of carboxy methyl cellulose (CMC) was tested on the agar plate. The highest hydrolysis capacity (HC value) was found in isolates ACSI (2.5), BDS31 (2.25), BFC8 (2.0) and FFC2 (1.02) respectively. The isolates of bacteria, actinomycetes and fungus were detected for filter paper activity for cellulase. Cellulase activity in bacterial isolates BDS31 and BFC8, actinomycetal isolate ACSI and fungal isolate FFC2 were 0.37, 0.36, 0.5 and 0.16 unit/ml, respectively. Cellulolytic microorganisms isolate ACSI, BDS31, BFC 8 and FFC2 were used to stimulate the germination of seeds of rice variety Phitsanulok 2 (Oryza sativa L.) and sweet corn (Zea mays saccharata) variety wanburi. The highest germination index value (GI) of rice root was 127.13 % when inoculated with bacteria isolate BDS31. Whereas, fungal isolate FFC2 could stimulate (GI 86.97 %) the germination of sweet corn root. 4 isolates of the cellulolytic microorganisms were studied on morphology under the compound microscope together with the analysis of DNA sequencing. The isolate of BFC8 was gram negative bacteria, rod shape and streptobacilli. Isolate BDS31 was gram positive bacteria, rod shape, endospore forming and brow color of colony. Isolate ACSI has straight spore chain, yellow color of colony and gray color of spore and isolate FFC2 was black spore and white hypha color. The data of DNA sequencing were identified as Cronobacter sakazakii (100 % similarity), Bacillus licheniformis (98 % similarity), Streptomyces thermodiastaticus (100 % similarity) and Thermomyces lanuginosus (100 % similarity) respectively.


P.967-972

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Effect of Crude Extracts from Ya Faek Lum (Vetiveria zizanioides (L.)
Nash) Leaves on Growth of Curvularia lunata (Wakker) Boedijn

ผลของสารสกัดหยาบจากใบหญ้าแฝกลุ่ม (Vetiveria zizanioides (L.)
Nash) ต่อการเจริญของเชื้อ Curvularia lunata (Wakker) Boedijn

รัฐพล ศรประเสริฐ

Extraction of Ya Faek Lum (Vetiveria zizanioides (L.) Nash) leaves, Suratthani strain with ethanol 95% solvent yielded a crude extracts which was characterized by a concentrated-sticky, dark green color and pleasant aroma with 1.47% yield. This research studied the effect of crude extracts against Curvularia lunata (Wakker) Boedijn. The crude extract at 20,000 ppm showed the best inhibiting effect against C. lunata (Wakker) Boedijn, following by 15000, 10000, 5000 and 0 ppm respectively which had inhibited percentile 34.02, 24.63, 22.43, 9.14 and 0.00 respectively. The minimum inhibitory concentration of crude extracts against of C. lunata (Wakker) Boedijn growth was equivalent to 0.125 mg/ml.


P.973-984

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Effect of Preservation Time, Light and Temperature of Cyanobacterial Extract, Phormidium angustissimum, on the Inhibit Seed Germination Ability of Chinese Cabbage

ผลของระยะเวลา แสง และอุณหภูมิในการเก็บรักษาสารสกัด จากไซยาโนแบคทีเรีย, Phormidium angustissimum ต่อความสามารถในการยับยั้งการงอกของเมล็ดผักกาดเขียวกวางตุ้ง

สุนีรัตน์ เรืองสมบูรณ์   ศักดิ์ชัย ชูโชติ และ ดุสิต เอื้ออำนวย

The effect of preservation time, light and temperature on stability of cyanobacterium; Phormidium angustissimum extract to inhibit the germination of bioassay seed, Brassica chinensis var. parachinensis Tsen & Lee, were studied. Cyanobacterial extracted by water and methanol were kept under different conditions; 4oC and 25oC with and without light for 0 to 15 days. The stability of cyanobacterial extracts to inhibit the germination of bioassay seed were tested at 0, 1, 2, 3, 5, 7, 9, 11, 13 and 15 days. At 0-11 days of preservation in all conditions, cyanobacterial extracted by methanol showed 100% inhibit the germination of bioassay seed. The inhibition efficiency was decreased when the preservation time increased. Cyanobacterial extracts preserve at 4oC had more inhibitory effect on the germination than at 25oC. Keeping the cyanobacterial extracts in the dark showed higher inhibitory effect on germination compared to that under the light.


P.985-995

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The Inhibitory Effects of Herbal Product, CHD-1 Raisin Tree on Methicillin-Resistant Staphylococcus aureus

ฤทธิ์การยับยั้งของผลิตภัณฑ์สมุนไพร CHD-1 Raisin Tree ต่อ Methicillin-Resistant Staphylococcus aureus และแบคทีเรียที่ทำให้ อาหารเน่าเสีย

สุบัณฑิต นิ่มรัตน์   จำลอง แสงส่ง   วีรสิทธิ์ ขำวผ่อง   พีรพัฒน์ สุพรรณพันธุ์ และ วีรพงศ์ วุฒิพันธุ์ชัย

In this study, the CHD-1 Raisin Tree, a herbal product which derived from plant called Raisin tree and produced in Republic of Korea, was investigated. The two types of product: tablet and capsule, were different for their antibacterial activity against two bacterial groups of Methicillin-resistant Staphylococcus aureus (MRSA) resistant to several antibiotics and spoilage bacteria isolated from dried seafood product (Bacillus coagulans, B. laterosporus, B. licheniformis, Staphylococcus caprae and S. hominis). The activities of two herbal product types were revealed by the agar well diffusion assay. The tablet type dissolved by distilled water showed effective growth inhibition against 5 strains of MRSA and spoilage bacteria with inhibition zone in a range of 10.00+-0.00 to 16.70+-0.12 mm and 13.30+-0.15 to 21.00+-0.10 mm, respectively. Whereas, product in capsule type showed no activity against all of the both test MRSA and spoilage bacteria. Therefore, the herbal product, CHD-1 Raisin Tree in tablet type was able to apply as a new effective drug in order to inhibit pathogenic bacteria, especially MRSA, and other bacteria for replacing antibiotics in future.


P.996-1007

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Comparison of Growth and Lead Accumulation of Sunflower and Sorghum in Lead Contaminated Soil

เปรียบเทียบการเจริญเติบโตและการสะสมตะกั่วของทานตะวันและข้าวฟ่างในดินที่ปนเปื้อนตะกั่ว

สายชล สุขญาณกิจ และ ธนวรรณ พาณิชพัฒน์

This research was conducted in the greenhouse to determine the concentration of lead accumulation in different parts of sunflower and sorghum planting in lead contaminated soil from Klity Village, Kanchanaburi province and comparison efficiency of lead translocation from soil into plants at harvesting of 35 days, 70 days and 105 days by considering BCF and TF. The results indicated that sunflower and sorghum accumulated lead to the highest concentration of 137.79±20.14 mg kg-1 and 80.73±12.52 mg kg-1 in 105 days, respectively. Both sunflower and sorghum were the highest lead accumulation in roots, stems and leaves, pericarps and seeds, respectively. The results showed the concentration of lead in seeds exceeded the standard for human consumption as food but did not exceed the standard as animal feed. The study of growth both sunflower and sorghum in lead contaminated and uncontaminated soil demonstrated the similar growth rate (P>0.05). The BCF was the highest efficiency of lead translocation from soils to accumulate in various parts of sunflower in 105 days and TF was the highest efficiency of lead translocation from roots to shoots of sorghum in 35 days.


P.1008-1018

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Utilization of Star Fruit Pomace Powder as Antioxidants Source in Salad Dressing

การใช้กากมะเฟืองผงเพื่อเป็นแหล่งของสารแอนตี้ออกซิแดนท์ในน้ำสลัด

ปรรัตน์ ศุภมิตรโยธิน

Salad dressing was fortified by dried star fruit pomace which is a good source of natural antioxidant. The purpose of this study was to determine an optimal condition of SFP drying as for maximum nutritional value. The research methodology has divided into 3 step 1) determining the physico-chemical properties star fruit at 4 ripening stages: dark green (DG), light green (LG), color break (CB), and ripe (R) 2) studying drying conditions (hot air, microwave, and sun drying) and 3) studying dried star fruit pomace powder adding in salad dressing (0-3% w/w). The CB-stages of star fruit showed the highest bioactive compounds: total phenolic content of 2985 mg Gallic acid/100 g FW, vitamin C of 21.6 mg/100 g, antioxidant activity of126.00 mol AEAC/g FW. Hot air drying at 50 °C for 180 min showed the best drying condition. The SFP powder contained total phenolic content of 7239.56 mg Gallic acid/100 g FW, vitamin C of 6.2 mg/100 g, antioxidant activity of 136.21 mol AEAC/g FW and water activity of 0.42. Salad dressing added with star fruit pomace powder contained high levels of total phenolic content, vitamin C and antioxidant activity. An optimum concentration of star fruit pomace powder was equal to 3% which retard rancidity, inhibit microorganism growth and increase antioxidant activity of salad dressing. This study demonstrated that star fruit powder may be used as a functional food ingredient for promoting health.


P.1019-1029

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Effects of Defatted Rice Bran on Properties of Natural Rubber Latex Foam

อิทธิพลของร้ำสกัดน้ำมันต่อสมบัติของฟองน้ำยางธรรมชาติ

ดริญญา มูลชัย และ อรุณศรี เอี่ยมรัมย์

The effects of defatted rice bran (DRB) as a filler for natural rubber latex foam on its properties were investigated. The contents of DRB which pass through a 150-mesh screen used were 0, 10, 20, 30 and 40 phr. The maximum content of DRB that can be used as a filler for natural rubber latex foam was 30 phr. It was found that increasing amount of DRB showed a difficulty of making natural rubber latex foam. Gellation time, density, indentation force and compression set of DRB-filled natural rubber latex foam increased with increasing amount of DRB. However, the properties of natural rubber latex foam filled with 10 phr of defatted rice bran passed the standard specification of latex foam rubber according to TIS 173-2519. It can be graded equivalent to RU 20 with indentation force of 2.08 kPa and compression set of 9.20%. In addition, the cost of rubber compound having 10 phr of DRB which passed through a 150-mesh screen is lower than that of non-filled rubber compound about 14 baht/kilogram.


P.1030-1042

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Comparison of Three RNA Extraction Methods for Gene Expression Analysis by RT-qPCR from Piperaceae Plants

การเปรียบเทียบการสกัดอาร์เอ็นเอสามวิธีเพื่อใช้ในการศึกษา การแสดงออกของยีนด้วยเทคนิค RT-qPCR จากพืชวงศ์พริกไทย

ชนนิษฏ์ ชูพยัคฆ์   กำไร วรนุช   รัตติกานต์ บัวเรือง และ อนุพันธ์ กงบังเกิด

Piperaceae plants are medicinal herbs that coexist with Thai society for a long time. Sesquiterpenes, the active components of those plants, have pharmacological interest. However, the complexity of molecular structure makes them still be expensive. Biotechnology should be the better choice for high quality and cheap chemical production. To achieve that goal, the regulation of biosynthetic gene expression needed to be studied that requires high quality of RNA. However, high phenolics and high chlorophyll contents in leaves obstructed RNA isolation from Piperaceae. The aim of this work was to obtain the efficient method for RNA isolation from P. betle, P. sarmentosum and P. retrofractum leaf. By comparison between different methods, RNeasy Plant Mini kit (QIAGEN), TRIzol
Reagent (Life technologies TM) and easy-RED TM RNA extraction (iNtRON Biotechnology), it was found that RNeasy Plant was the most efficient method. RNA isolated from RNeasy Plant method was high quality, integrity and equal amount from three tested plant species. Afterwards, RNeasy Plant - isolated RNA was further used for gene expression analysis using RT-qPCR technique. It was demonstrated that the RNA could be used as a template for Actin and Sesquiterpene synthase transcript amplification. Taken together, RNeasy Plant Mini kit was the ideal method for isolation of RNA from Piperaceae plants using for gene expression analysis and molecular study.


P.1043-1056

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Potential of Bacteria Isolated from a Dye Contaminated Site on Decolorization of Reed Dyeing Wastewater

ศักยภาพของแบคทีเรียที่แยกได้จากแหล่งปนเปื้อนสีย้อม ในการลดความเข้มสีในน้ำเสียที่ได้จากการย้อมกก

นิตยา เลี้ยงถนอม   และ   จิรภัทร จันทมาลี

The release of colored waste effluents from reed mat production may cause negative impact on water quality. This study aimed to isolate dye-degrading bacteria from reed manufacturing areas in Chanthaburi as well as to study the efficiency of the selected strains on removal of reed dye. At first, eighteen strains of dye-degrading bacteria were isolated by dye enrichment culture techniques. After that, the growth on Mineral Salt Medium containing 1,000 mg/l synthetic reed dye as sole carbon source and the exopolysaccharide (EPS) production ability of each isolate were determined. EPS has been shown to absorb dye. The results showed that isolate A1 and A8 grown well on the tested medium and had high EPS’s producing ability (82-85%). The identification results found that A1 and A8 were belonged to genus Serratia and Klebsiella, respectively. After that, the dye removal efficiency of the selected strains was determined in MS containing 100 mg/l red dye. The treatments were incubated with shaking (150 rpm) at room temperature for 14 days. Serratia sp. A1, the most effective strain could reduce the color intensity at 11.56%. Therefore, A1 was selected to test for its efficiency in reducing the intensity of other reed dyes. This bacterial strain could reduce the color intensity of yellow and mixed dye at 10.84 and 12.16%, respectively. Finally, Serratia sp. A1 was used to treat real reed mat effluent. The bioaugmentation flasks containing 100 mg/l dye and inoculum (OD600 = 0.1) showed the good activity of reducing dye color intensity at 12.89% after 8-day incubation.


P.1057-1065

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Screening and Identification of Cellulase Producing Thermotolerant Fungi as Compost Starter

การคัดเลือกและการจัดจ าแนกเชื้อราทนร้อนที่ผลิตเอนไซม์เซลลูเลส เพื่อการผลิตเป็นหัวเชื้อปุ๋ยหมัก

Aree Rittiboon   Marisa Jatupornpipat and Piroonporn Srimongkol 1

The purposes of this research were to screen and identify the fungi isolated from soil having high cellulase activities for producing a compost starter. Thirty-nine isolates of fungi from soil were isolated. The primary screening of the fungi was done in the solid state medium with cellulose as the carbon source and incubated at 45°C. The ratio of the clear zone and the colonial sizes were determined. The secondary screening was done in liquid state fermentation with cellulose as the carbon source and the activities of the Carboxymethylcellulase (CMCase) were measured. The CMCase activity of the isolate FA68 was the highest and followed by that of the isolate FA50 with the enzyme activity (0.17 and 0.13. IU/ml). The isolates FA68 and FA50 were then cultivated on agricultural wastes, in solid state cultivation. The isolate FA68 grew well at 45ºC for 7 days and produced higher levels of CMCase activities compared to those of the isolate FA50 with activity 0.23 and 0.22 IU/g, respectively. When both the fungal isolates were cultivated and studied the growth in sorghum grains in order to produced as compost starter, it was found that at 37 ºC and 80 % initial moisture content, the isolates FA68 and FA50 had the highest sporulation rates of 10.27 and 9.46 log spores/g sorghum grains after 6 and 5 days incubation, respectively. Both the isolates FA68 and FA 50 were identified to be the genus Aspergillus


P.1066-1073

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Period Change of V1162 Orionis Variable Star

การเปลี่ยนคาบของดาวแปรแสง วี 1162 โอไรโอนิส

รณกฤต รัตนมาลา และ วันธนา ศิลปวิลาวัณย์

The study of period change of variable star V1162 Orionis was conducted at Princess Sirindhorn Observatory, Chiang Mai University. The observation was done via CCD photometer in blue (B) and visible (V) bands. The data were employed to analyze and plot light curves. The period change analysis by O-C diagram was shown the decreasing rate at 11.2886x10-12 day/cycle
(4.527 msec/year). The result likely showed that the V1162 Orionis was collapsed.